About iPhos
iPhos Facilitated Comprehensive Phosphoproteome Investigation
The Proposed Experiment Flow
The sketch plot of the overall iPhos-facilitated phosphorylated peptide identification and quantification work-flow. In this figure, the input and output file formats for different modules are shown in gray.
The iPhos Toolkit
The iPhos toolkit includes one assister and three modules.
- The iPhos Peak Extraction Assister : to automate the peak extraction for multiple liquid chromatography mass spectrometry (LC-MS) runs for users.
- iPhos Module-1 : to process the peak lists extracted from the LC-MS analyses derived from phosphorylated and dephosphorylated samples to mine out potential phosphorylated peptide signals based mass shift caused by the loss of some multiples of phospho-groups.
- iPhos Module-2 : to provide customized inclusion lists with peak retention time windows for subsequent targeted LC-MS/MS experiments.
- iPhos Module-3 : to link the peptide identifications from protein search engines to the quantification results from pattern-based label-free quantification tools.