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About iPhos

iPhos Facilitated Comprehensive Phosphoproteome Investigation

The Proposed Experiment Flow

flow The sketch plot of the overall iPhos-facilitated phosphorylated peptide identification and quantification work-flow. In this figure, the input and output file formats for different modules are shown in gray.

The iPhos Toolkit

The iPhos toolkit includes one assister and three modules.

  1. The iPhos Peak Extraction Assister : to automate the peak extraction for multiple liquid chromatography mass spectrometry (LC-MS) runs for users.
  2. iPhos Module-1 : to process the peak lists extracted from the LC-MS analyses derived from phosphorylated and dephosphorylated samples to mine out potential phosphorylated peptide signals based mass shift caused by the loss of some multiples of phospho-groups.
  3. iPhos Module-2 : to provide customized inclusion lists with peak retention time windows for subsequent targeted LC-MS/MS experiments.
  4. iPhos Module-3 : to link the peptide identifications from protein search engines to the quantification results from pattern-based label-free quantification tools.